View and buy high quality Cyanine 5, SE from Tocris Bioscience. *Please select more than one item to compare. For FITC and Cy5 derivatization, Fmoc-β-Ala was deprotected with piperidine in N-methylpyrrolidone (NMP) and then reacted with FITC or Cy5 NHS ester (Lumiprobe) (3 eq relative to resin-bound amine) Site Use Terms Cy5 is intensely fluorescent and highly water soluble. Since NHS-esters react readily only with aliphatic amine groups, which nucleic acids lack, nucleotides have to be modified with aminoallyl groups. Sigma-Aldrich Products are sold exclusively through Sigma-Aldrich, Inc. Cy®5 Mono NHS Ester has been used in microarray experiments. In stock 5mg $138.00. Cy5 dyes are one of … Fax: +49 511 16596815 Germany, Phone: +49 511 16596811 1-858-677-9432. sales@axispharm.com. Cyanine5.5 NHS ester is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides, an analog of Cy5.5® NHS ester. It has been shown to be useful as fluorescent label for biological compounds. It is often a reagent of choice for protein and peptide l. ADC Technologies & Analytical Services & Worldwide Leading PEG Supplier. Cyanine5 NHS ester can replace activated esters of Cy5ィ, Alexa Fluor 647, DyLight 649 for all applications. Cy5.5 is intensely fluorescent and highly water soluble. Cy5 NHS Ester monosulfo $99.00 Save $-99. Cyanine 5 (Cy5®) dyes are used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. The labeled peptide 15 was separated using size exclusion chromatography and lyophilized to yield a blue amorphous product. The NHS ester (or succinimidyl ester) of Alexa Fluor® 647 is the most popular tool for conjugating this dye to a protein or antibody. This regent is especially useful for the labeling of proteins which It is often a reagent of choice for protein and peptide l. ADC Technologies & Analytical Services & Worldwide Leading PEG Supplier. Compare Products: Select up to 4 products. Analog of Cy5.5 ® NHS ester. Product Name: Cy5 NHS Ester: Formula: C 36 H 42 N 3 O 10 S 2? Size Price Stock Qty; 10mM (in 1mL DMSO) $272.00. Cy ® 5.5 Mono NHS Ester. For FITC and Cy5 derivatization, Fmoc-β-Ala was deprotected with piperidine in N-methylpyrrolidone (NMP) and then reacted with FITC or Cy5 NHS ester (Lumiprobe) (3 eq relative to resin-bound amine) and triethylamine (3 eq) in dimethylformamide overnight. Cy5.5 is a far-red (and near-infrared) emitting dye which is ideal for fluorescence measurements where background fluorescence is a concern. This regent is especially useful for the labeling of proteins which denaturate in the presence of organic co-solvents, as well as for proteins with low solubility. Determine volume of reaction mixture. Cy5.5-NHS ester is a near-infrared fluorescence dye able to dissolve in DMF and DMSO with the excitation maximum and emission maximum around 675 nm and 693 nm respectively [1]. Cy5-NHS Ester. Email: [email protected], Delivery dates and prices will be calculated based on your ZIP. This reagent is ideal for very cost-efficient labeling of soluble proteins, as well as all kinds of peptides and oligonucleotides. This dye requires a small amount of organic co-solvent to be used in labeling reactions. 1-858-677-9432. sales@axispharm.com. This reagent can be used to attach Cy5 fluorophore to proteins and peptides through lysine residues. Email: [email protected], (Russia and CIS) Also suitable for in vivo near-infrared (NIR) imaging experiments. Cy5 NHS ester, 5 This reagent is ideal for very cost-efficient labeling of soluble proteins as well as all kinds of peptides and oligonucleotides. Cy5 Bis NHS Ester contains two reactive groups on each dye molecule. Cy5 dye (also sold under Sulfo-Cyanine5 name) is one of the most popular far-red fluorescent dyes. Cyanine5 fluorophore is compatible with various instrumentation including many fluorescent microscopes, imagers, scanners, and fluorescence readers. Since NHS-esters react readily only with aliphatic amine groups, which nucleic acids lack, nucleotides have to be modified with aminoallyl groups. 8:00 - 19:00 GMT+3 (Moscow), Kotsyubinskogo 4, bld. Excitation. Description: Cy5.5 NHS Ester (also sold under sulfo-Cyanine5.5 NHS Ester name) is a bright and photostable near-IR dye that is spectrally similar to Alexa Fluor® 680, DyLight® 680, and IRDye® 680 dye. CyDyes are suitable for use in applications such as fluorescence resonance energy transfer (FRET), time resolved- fluorescence resonance energy transfer (TR-FRET), and fluorescence polarization (FP). The labeling can be performed on any scale from nanomols to dozens of grams. Bioz Stars score: 89/100, based on 12 PubMed citations. Cy5 NHS ester 592 Da Cy5.5 NHS ester 723 Da Alkyne STP ester 352 Da Azide NHS ester 254 Da Please note that molar weights of reagents produced by other vendors may vary. SKU: T01127-1mg. Cy5.5 NHS Ester is completely water-soluble and often a reagent of choice for labeling antibodies, peptides, and proteins that are sensitive toward organic co-solvents. Cy5.5 is intensely fluorescent and highly water soluble. CyDye Value Packs (Mono-Reactive NHS Ester) contain derivatized CyDye fluors that carry only one reactive group on each dye molecule for accurate labeling of amine functional groups. Cy5 NHS ester 592 Da Cy5.5 NHS ester 723 Da Alkyne STP ester 352 Da Azide NHS ester 254 Da Please note that molar weights of reagents produced by other vendors may vary. 8:00AM - 5:00PM CE(S)T, Feodor-Lynen-Strasse 23 For Cy5 labeling, the purified peptide was dissolved in DMSO, Cy5-NHS ester was added, and the reaction was monitored using analytical HPLC at 280 nm. 121351 Moscow For the labeling of various amine-containing molecules in aqueous phase, no organic co-solvent is needed. Sorry we cannot compare more than 4 products at a time. *Please select more than one item to compare, 14 Cy5 conjugates are very bright, photostable, pH insensitive and produce minimal autofluorescence of biological specimens in … Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak. Cy5.5 is a far-red (and near-infrared) emitting dye which is ideal for fluorescence measurements where background fluorescence is a concern. It is also suitable for in vivo NIR imaging experiments. In microarray experiments DNA or RNA is labeled with either Cy3 or Cy5 that has been synthesized to carry an N-hydroxysuccinimidyl ester (NHS-ester) reactive group. Provide Content Correction We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Cy5 dye (also sold under Sulfo-Cyanine5 name) is one of the most popular far-red fluorescent dyes. Z-Cy5 NHS Ester Amine mono-reactive zwitterionic and pI balancing Cy5 dye (Z-Cy5 NHS Ester) with titratable side chain tertiary amine. Upon receipt, Cyanine 5 monosuccinimidyl ester [equivalent to Cy5® NHS ester] should be stored at < - 15 °C, dessicated and protected from light. Facebook Twitter linkedin. This reagent can be utilized to produce Cyanine-labeled biomolecules for subsequent use such as in vivo research, and drug design related experiments. Enzo cyanine 5 NHS-ester is a reactive, water-soluble fluorescent dye that provides bright red signal. Properties. The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Cyanine5.5 NHS ester is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. 30625 Hannover Hunt Valley, Maryland 21030 The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Fax: +1 888 973 6354 Sulfo-Cy5 conjugates are recommended for detection of moderate-to-high abundance targets. Keep in touch with us having discounts and technical information in your inbox. It is a red dye which spectrally lies between Texas Red® (NHS Ester) and Cy5™. 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